Interactions of Protonated Guanidine and Guanidine Derivatives with Multiply Deprotonated RNA Probed by Electrospray Ionization and Collisionally Activated Dissociation

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Interactions of Protonated Guanidine and Guanidine Derivatives with Multiply Deprotonated RNA Probed by Electrospray Ionization and Collisionally Activated Dissociation

Interactions of ribonucleic acid (RNA) with guanidine and guanidine derivatives are important features in RNA-protein and RNA-drug binding. Here we have investigated noncovalently bound complexes of an 8-nucleotide RNA and six different ligands, all of which have a guanidinium moiety, by using electrospray ionization (ESI) and collisionally activated dissociation (CAD) mass spectrometry (MS). T...

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The effects of the n-alkyl derivatives of guanidine on the frog neuromuscular junction were studied using the two-microelectrode voltage clamp and other electrophysiological techniques. Methyl-, ethyl-, and propylguanidine stimulated the nerve-evoked release of transmitter. However, amyl-and octylguanidine had no apparent presynaptic action. All of the derivatives blocked the postsynaptic respo...

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The Antiviral Action of Polyhexamethylene Guanidine Derivatives

Although the PHMG (polyhexamethylene guanidine) and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG derivatives has been established firstly in this study. The antiviral protection was also exhibited after 15 min pretreatment of different cell cultures with low-concentration of PHMG sal...

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Surface-induced dissociation of multiply protonated peptides.

We report here surface-induced dissociation spectra of three multiply charged peptides: doubly protonated angiotensin I, doubly protonated renin substrate, and triply protonated melittin. For comparison, the collision-activated dissociation spectra of renin substrate and melittin are also presented. The spectra show that surface-induced dissociation provides structural information on multiply c...

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AKAGUCHI'S test for arginine depends on the production of a pink or red colour when certain guanidine derivatives are treated with a-naphthol and sodium hypochlorite in alkaline solution (Sakaguchi, 1925). This test had been familiar to biochemists for 19 years before it was independently adapted to histochemical use in the same year by Serra (1944) and myself (1944). Our results were published...

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ژورنال

عنوان ژورنال: ChemistryOpen

سال: 2017

ISSN: 2191-1363

DOI: 10.1002/open.201700143